Figure 5.

Soluble β-glucans block the ability of 2A11 to detect Dectin-1 on Tg-elicited peritoneal macrophages. Flow cytometric analysis of the surface levels of Dectin-1 and CR3 before and after the addition of glucan phosphate (GluP) or laminarin (Lam), with or without brief warming at 37°C to induce receptor internalization. Cells were stained with isotype-control (IgG2b) or receptor-specific mAbs, in the presence and absence of the exogenous glucans, as indicated above the panels. No difference in 2A11 staining was observed when the cells were warmed without glucan phosphate or laminarin (unpublished data).