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. 2002 Aug 5;196(3):359–368. doi: 10.1084/jem.20011838

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Validation of HA-1 expression in human primary tumors. (A) Evaluation of histogenetic markers by cDNA array analysis. Six microdissected areas that were HA-1⁺ by PCR were hybridized on a small array containing histogenetic markers. PN5-1, PN5-2, and PN5-3 were positive for CD45 in gene-specific PCR, whereas PN18-3, PN21-1, and PN22-3 were CD45⁻. Cytokeratin or MAGE transcripts represent epithelial or tumor-specific transcripts, whereas CD markers indicate leukocytic origin. ACTB and UB are housekeeping genes and GFP is the negative control. The gray shades represent the signal intensity, from light gray (weak signal) to black (strong signal). (B) CGH results of five of the six areas shown in A. The chromosome arms are given with green indicating a chromosomal gain and red indicating a chromosomal loss. The aberrations prove that the isolated areas contained at least 50% tumor cells.

Figure 3. — Validation of HA-1 expression in human primary tumors. (A) Evaluation of histogenetic markers by cDNA array analysis. Six microdissected areas that were HA-1⁺ by PCR were hybridized on a small array containing histogenetic markers. PN5-1, PN5-2, and PN5-3 were positive for CD45 in gene-specific PCR, whereas PN18-3, PN21-1, and PN22-3 were CD45⁻. Cytokeratin or MAGE transcripts represent epithelial or tumor-specific transcripts, whereas CD markers indicate leukocytic origin. ACTB and UB are housekeeping genes and GFP is the negative control. The gray shades represent the signal intensity, from light gray (weak signal) to black (strong signal). (B) CGH results of five of the six areas shown in A. The chromosome arms are given with green indicating a chromosomal gain and red indicating a chromosomal loss. The aberrations prove that the isolated areas contained at least 50% tumor cells.