FIG. 2.

Spo0J-GFP levels in IPs and whole-cell lysates from several B. subtilis strains. In all samples, cross-links made by the DSP cross-linker were cleaved with DTT before loading into an SDS-polyacrylamide gel. Immunoprecipitation was carried out with protein A-agarose beads coupled with anti-Soj rabbit antibody. Spo0J-GFP was detected by Western blotting with anti-GFP monoclonal antibody and horseradish peroxidase-conjugated second antibody. Lanes 1 to 3, IPs from wild-type (CRK6000), spo0J-gfp (NIS6072), and Δsoj spo0J-gfp (NIS6076) cells, respectively; lanes 4 and 5, whole-cell lysates from spo0J-gfp and Δsoj spo0J-gfp cells, respectively.