FIG. 4.

Analysis of replication initiation in several soj and spo0J mutants by flow cytometry. DNA histograms were obtained by flow cytometry for the following strains: soj⁺ spo0J⁺ strain NIS6080, sojG12V strain NIS6081, sojK16Q strain NIS6082, spo0J14 strain NIS6083, and spo0J17 strain NIS6084. The cells were grown in the presence of 50 μM IPTG because expression of both the soj and spo0J genes is controlled by the IPTG-inducible spac promoter in these mutant strains and at this IPTG concentration, the levels of both proteins were nearly comparable to those in the wild-type strain.