TABLE 1.
B. subtilis strains
| Strain | Relevant genotype | Reference, source, or constructiona |
|---|---|---|
| CRK6000 | purA16 metB5 hisA3 guaB | 36 |
| NIS6072 | CRK6000 spo0J-gfp | 19 |
| NIS6074 | CRK6000 spo0J::ermC | 26 |
| NIS6075 | CRK6000 Δsoj | Kobayashib |
| NIS6076 | CRK6000 Δsoj spo0J-gfp | pSM5072 (19) → NIS6075 (Cp) |
| NIS6077 | CRK6000 Δ(soj-spo0J)::tet | pYO3 → CRK6000 (Tc) |
| NIS6078 | CRK6000 amyE::Pspac-soj | pYO4 → CRK6000 (Cp) |
| NIS6079 | CRK6000 amyE::Pspac-soj-spo0J | pYO5 → CRK6000 (Cp) |
| NIS6080 | CRK6000 soj-spo0J::tet amyE::Pspac-sojG12V-spo0J | pYO5 → NIS6076 (Cp, Tc) |
| NIS6081 | CRK6000 soj-spo0J::tet amyE::Pspac-sojG12V-spo0J | pYO31 → NIS6076 (Cp, Tc) |
| NIS6082 | CRK6000 soj-spo0J::tet amyE::Pspac-sojK16Q-spo0J | pYO32 → NIS6076 (Cp, Tc) |
| NIS6083 | CRK6000 soj-spo0J::tet amyE::Pspac-soj-spo0J14 | pYO33 → NIS6076 (Cp, Tc) |
| NIS6084 | CRK6000 soj-spo0J::tet amyE::Pspac-soj-spo0J17 | pYO34 → NIS6076 (Cp, Tc) |
a
The arrows indicate transformation. The donor plasmid DNA (linear form except pSM5072) and the recipient strain are indicated on the left and right of each arrow, respectively; the antibiotics used for selection are indicated in parentheses (Cp, chloramphenicol; Tc, tetracycline).
b
K. Kobayashi, unpublished data. An in-frame deletion (from codon 22 to codon 135) was introduced into soj.