TABLE 2.

Various parameters for cell division and replication initiation in B. subtilis soj and spo0J mutants

Strain	Avg cell length (relative)a	Avg no. of origins per cellb	Amt of mass per origin (relative)c	DNA/protein ratiod
CRK6000 (wild type)	1.00	4.5 ± 0.1	1.00	0.041 ± 0.002
NIS7064 (spo0J::ermC)	1.17	8.0 ± 0.1	0.67	0.060 ± 0.001
NIS6077 [Δ(soj-spo0J)]	1.03	5.1 ± 0.2	0.92	0.044 ± 0.001
NIS6078 (Pspac-soj) + 1 mM IPTG	1.16	7.0 ± 0.2	0.75	0.060 ± 0.001
NIS6079 (Pspac-soj-spo0J) + 1 mM IPTG	1.20	4.3 ± 0.1	1.27	0.034 ± 0.002

^aThe average cell length for each strain was obtained by examining 300 cells and is expressed relative to the value for the wild-type cell population.

^bThe average number of replication origins per cell was determined from each DNA histogram of flow cytometry shown in Fig. 1A. The same experiment was repeated three times to obtain the average and standard deviation.

^cThe average cell length was divided by the average number of oriCs per cell to obtain the amount of mass per origin and is expressed relative to the value for the wild type.

^dAverage ± standard deviation obtained from three or four independent experiments.