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. 2003 Jun 16;197(12):1657–1666. doi: 10.1084/jem.20021546

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — CD36 is expressed on adult mouse microglia and mediates fAβ-induced ROS production. (A–F) Neonatal mouse microglia (A and D), adult mouse microglia (B and E), or peritoneal mouse macrophages (C and F) were stained with mAb to mouse CD36 or isotype-matched control followed by FITC-labeled secondary antibody, visualized by fluorescence microscopy, and digitally photographed. (G) mRNA from adult mouse microglia was harvested for Q-PCR using probes specific for SR-A, SR-B1, SR-F, CD36, and Lox-1. (H) Adult microglia from CD36^+/+ and CD36^−/− mice were cultured on multispot slides and incubated with 10 μg/spot fAβ or revAβ for 1 h. ROS production was assayed by the NBT reduction method, which produces visible dark blue formazan deposits. The amount of deposited formazan was quantified using digital video microscopy and Scion image analysis software.