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. 2003 Jun 16;197(12):1657–1666. doi: 10.1084/jem.20021546

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Intracerebral fAβ-induced recruitment of microglia is CD36 dependent. CD36^+/+ (A and B) or CD36^−/− (C and D) mice stereotaxically microinjected with 2 μg fAβ (A and C) or revAβ (B and D) into opposite hemispheres. 48 h later, mice were killed and 20-μm frozen sections were stained with F4/80 (green) and DAPI (blue; original magnification, ×4). The fluorescence intensity of F4/80 staining representing the number of microglia per injection site was quantified by digital imaging analysis (E). A higher magnification image showing details of cytoplasmic F4/80 and nuclear DAPI staining is shown in F. Each data point is the average ± SEM of five different sections per mouse and is a representative of five to seven mice per genotype with similar results. *, P < 0.005.