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. 2003 Jun 16;197(12):1787–1791. doi: 10.1084/jem.20022078

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Detection of wild-type, Asp299Gly, and Thr399Ile alleles in the human TLR4 gene. Genomic DNA of individuals recruited into the current study was amplified according to the method of Lorenz et al. (reference 4). PCR products were digested with the enzymes NcoI (299 allele) or HinfI (399 allele). Lanes 1 and 14, 100 base pair marker. Lanes 2–7, RFLP of the 299 section of TLR4 gene from three wild-type donors and three heterozygotes. Lanes 8–13, RFLP of the 399 section of TLR4 gene from three wild-type donors and three heterozygotes. Top band represents presence of wild-type allele, bottom band indicates presence of mutant allele. Genotypes were confirmed by sequencing.