Table I.
Comparison of Homozygous Wild-Type and Wild-Type/Asp299Gly,Thr399Ile Heterozygote TLR4-bearing Monocyte Recognition of Various LPS
| Stimulus | Minimum concentration activating wild-type monocytes (ng/ml)a |
Minimum concentration activating TLR4+/− monocytes (ng/ml)a |
|---|---|---|
| E. coli LPS | 0.01 | 0.01 |
| N. meningitidis LPS | 0.1 | 0.1 |
| B. fragilis LPS | 1 | 1 |
| P. gingivalis LPS | 10 | 10 |
| Y. pestis LPS | 10 | 10 |
| C. trachomatis LPS | 100 | 100 |
| P. aeruginosa LPS | 100 | 100 |
| S. aureus LTA | 100 | 100 |
Homozygous wild-type or Asp299Gly/Thr399Ile heterozygous (TLR4+/−) human monocytes were challenged with 10-fold serial dilutions of E. coli, N. meningitidis, Y. pestis, C. trachomatis, B. fragilis, P. aeruginosa, P. gingivalis LPS, or S. aureus LTA or medium alone.
a
Minimum concentration represents the lowest concentration at which individual stimuli induce production of significantly more IL-1β (P < 0.05) than cells incubated with medium alone.