Figure 5.

LTβR mediated activation of RelB through NIK. (A) ICAM-1 induction by LTβR stimulation. Fibroblasts from the kidneys of RelB ^{+ / +} or RelB ^{− / −} mice were stimulated with an agonistic α-LTβR mAb (2 μg/ml) for 24 h. Cell surface levels of ICAM-1 were determined by flow cytometry. Histograms representing ICAM-1 levels before (thin line) and after stimulation (bold line) are shown. One representative example of three independent experiments is shown. (B) NF-κB/Rel binding activities in wild-type and aly/aly mice after LTβR ligation. MEFs from C57BL/6 and aly/aly mice were stimulated with an α-LTβR mAb (2 μg/ml) for 8 h. Nuclear extracts were prepared from unstimulated and LTβR triggered cells. Extracts were incubated with a palindromic κB-binding site as described in Materials and Methods. The results from addition of specific anti-sera against RelA (p65) and RelB are indicated at the bottom. One representative experiment of three is shown.