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. 2003 Jun 16;197(12):1635–1644. doi: 10.1084/jem.20030215

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — T cell responses of GAD65 transgenic animals against GAD65 and its peptide epitopes. (A) Percent of CD4⁺ cells from lymph nodes (draining lymph nodes: open bars; mesenteric lymph nodes: gray bars) of NOD control and GAD65tg. mice 8 d after immunization with GAD65 in CFA as measured by FACS^® analysis. Percent values of background tetramer staining (animals just immunized with CFA [0.034–0.062%]) were subtracted from each sample (n = 4). (B) Representative FACS-plot of I-A^g7-p286 tetramer staining of lymph node cells from NOD control mice (B) and GAD65tg. mice (C) immunized with GAD65 in CFA (top two panels) or CFA alone (bottom two panels). (D) Number of IFN-γ–secreting cells from draining lymph nodes of NOD-control (open bars) and GAD65tg. mice (gray bars) after immunization with GAD65 as determined by ELISPOT analysis.

Figure 3. — T cell responses of GAD65 transgenic animals against GAD65 and its peptide epitopes. (A) Percent of CD4⁺ cells from lymph nodes (draining lymph nodes: open bars; mesenteric lymph nodes: gray bars) of NOD control and GAD65tg. mice 8 d after immunization with GAD65 in CFA as measured by FACS^® analysis. Percent values of background tetramer staining (animals just immunized with CFA [0.034–0.062%]) were subtracted from each sample (n = 4). (B) Representative FACS-plot of I-A^g7-p286 tetramer staining of lymph node cells from NOD control mice (B) and GAD65tg. mice (C) immunized with GAD65 in CFA (top two panels) or CFA alone (bottom two panels). (D) Number of IFN-γ–secreting cells from draining lymph nodes of NOD-control (open bars) and GAD65tg. mice (gray bars) after immunization with GAD65 as determined by ELISPOT analysis.