Figure 2.
Response of transgenic T cells to MOG 35–55 and MOG protein. Splenocytes from unimmunized mice were cultured with indicated concentrations of MOG 35–55 peptide (A) or recombinant protein MOG (B). T cell proliferation was determined by [3H]thymidine incorporation in triplicate wells in a 72-h proliferation assay. The mean cpm's in the presence of 50 μg/ml OVA 323–336 control peptide were 12,401 for TCR transgenic mice and 14,278 for the control littermates. Splenocytes produce IFN-γ in response to indicated concentrations of peptide MOG 35–55 (C). IFN-γ was measured on supernatants harvested 48 h after activation by ELISA. 2D2 Tg, 2D2 transgenic; NT, nontransgenic.