FIG. 3.

Genomic maps of VP16T and VP16C. ORFs are color coded based on the BLAST E-values and are labeled largely based on the BLAST hits shown in Table 2. BLAST hits returning hypothetical proteins or weak E-values (≤1e⁻²) are shown as unlabeled color-coded ORFs. Gray ORFs do not exhibit homology to GenBank entries. An ORF labeled with a solid diamond is a VP16 ORF that was identified by the neural network as an ORF that encodes a possible capsid protein. The amino acid sequences of VP16T proteins were found in VP16T ORF sequences labeled TS1 (ANELATGWVQ), TS2 (LIKLT), and TS3 (ADRHIL). Similarly, amino acid sequences of VP16C proteins were encoded in the VP16C ORF sequences labeled CS1 (ANELATGWVQ) and CS2 (LVKLS). ORFs encoding sequences shorter than 70 amino acids are not included in the map for ease of display, but all ORFs are listed in Table 2.