FIG. 4.

 SDS-polyacrylamide gel electrophoresis analysis of VP16C and VP16T phage capsid proteins. TS and CS are structural proteins from the turbid- and clear-plaque phages, respectively. Twenty or 30 μl of phage lysate was mixed with Laemmli buffer containing SDS, boiled for 10 min, and loaded on a 4 to 20% polyacrylamide gradient gel that was electrophoresed with Tris-glycine runing buffer. Color-coded molecular weight markers were electrophoresed in parallel.