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. 2003 May 5;197(9):1083–1091. doi: 10.1084/jem.20021752

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Costimulation of PD-1^−/− T cells by B7-H1 and B7-DC mutants. Purified T cells from PD-1–deficient B6 mice were stimulated with immobilized wild-type or mutants of B7-H1 (a) and B7-DCIg (b) at 5 μg/ml in the presence anti-CD3 mAb at indicated concentrations. Human Ig (hIg) was used as negative control. The proliferation of T cells was determined by incorporation of 1 μCi/well of ³H-TdR during the last 12 h of the 3-d culture. Data depict one representative experiment of three.