TABLE 2.
Classification of CheY active-site positions according to functional tolerance of amino acid substitutions
| Level of tolerance | CheY position | Substitutions giving
|
|
|---|---|---|---|
| Some CW activity (reference or source) | No CW activity (reference or source) | ||
| Tolerant | Asp13 | Arg (7) | Ala, Cys, Gln, Gly, His, Leu, Phe, Tyr (7) |
| Lys (8) | Glu (8) | ||
| Asn, Met, Ser, Thr (32) | |||
| Phe14 | His, Arg (Table 1) | Asp, Glu, Lys (Table 1) | |
| Tyr (7) | |||
| Leua | |||
| Trp58 | Arg, His, Lys (Table 1) | Asp, Glu (Table 1) | |
| Ile, Pheb | |||
| Asn59 | Arg, His, Lys (Table 1) | Asp, Glu (Table 1) | |
| Ala (38) | |||
| Gln, Sera | |||
| Met60 | His (Table 1) | Asp, Glu, Lys, Arg (Table 1) | |
| Pro61 | Arg, Lys (Table 1) | Asp, Glu, His (Table 1) | |
| Ser, Thra | |||
| Glnb | |||
| Partially tolerant | Asp12 | Glu (8) | Arg, His (Table 1) |
| Gly (7) | |||
| Asn, Lys (8) | |||
| Alab | |||
| Thr87 | Ala, Cys, Ser (3) | Arg, Asp, His (Table 1) | |
| Glu, Lys (3) | |||
| Ile (13) | |||
| Glya | |||
| Intolerant | Met17 | Arg, Asp, Glu, His, Lys (Table 1) | |
| Ser, Thra | |||
| Glnb | |||
| Asp57 | Arg, His (Table 1) | ||
| Ala, Gln, Leu, Ser (7) | |||
| Asn, Glu, Lys (8) | |||
| Cys (36) | |||
| Val86 | Arg, Asp, Glu, His, Lys (Table 1) | ||
| Ala88 | Arg, Asp, Glu, His, Lys (Table 1) | ||
| Met, Vala | |||
| Glnb | |||
| Lys109 | Asp (Table 1) | ||
| Cys, Gln, Glu, Gly, His, Leu, Met, Phe, Pro, Ser, Thr, Tyr, Val (7) | |||
| Arg (27) | |||
a
J. G. Smith et al., unpublished data.
b
R. B. Bourret, unpublished data.