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. 2002 Nov 18;196(10):1321–1333. doi: 10.1084/jem.20012135

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Immunohistochemical staining of the pan-T cell marker CD3 (a–e; brown), as well as CD4⁺ (f–j; brown), CD8⁺ (k–o; red) T cells, and F4/80⁺ (p–t; brown) macrophages in sections from grafts transplanted into isogeneic (a, f, k, and p), allogeneic WT (b, g, l, and q), iNOS^−/− (c, h, m, and r), eNOS^−/− (d, i, n, and s) recipients, and L-NIL–treated WT (e, j, o, and t) recipients 3 wk after transplantation. Quantitative analysis of mononuclear cell infiltrates by FACS^®, shown as absolute numbers of recovered CD3⁺ CD4⁺ and CD3⁺CD8⁺ T cells (u), and F4/80⁺ macrophages (v) in each graft from each group. Bar, 50 μm; *, P < 0.05 versus allografts from WT recipients; #, P < 0.05 versus allografts from iNOS^−/− recipients; †, P < 0.05 versus allografts from eNOS^−/− recipients.

Figure 2. — Immunohistochemical staining of the pan-T cell marker CD3 (a–e; brown), as well as CD4⁺ (f–j; brown), CD8⁺ (k–o; red) T cells, and F4/80⁺ (p–t; brown) macrophages in sections from grafts transplanted into isogeneic (a, f, k, and p), allogeneic WT (b, g, l, and q), iNOS^−/− (c, h, m, and r), eNOS^−/− (d, i, n, and s) recipients, and L-NIL–treated WT (e, j, o, and t) recipients 3 wk after transplantation. Quantitative analysis of mononuclear cell infiltrates by FACS^®, shown as absolute numbers of recovered CD3⁺ CD4⁺ and CD3⁺CD8⁺ T cells (u), and F4/80⁺ macrophages (v) in each graft from each group. Bar, 50 μm; *, P < 0.05 versus allografts from WT recipients; #, P < 0.05 versus allografts from iNOS^−/− recipients; †, P < 0.05 versus allografts from eNOS^−/− recipients.