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. 2002 Nov 18;196(10):1321–1333. doi: 10.1084/jem.20012135

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Apoptotic cells were detected using the in situ TUNEL labeling method (a–d; arrows) and further corroborated immunohistochemically by colocalizing caspase-3 expression in the same cells in adjacent sections (e–h; arrows). Double staining for apoptotic CD8⁺ T lymphocytes (i–l; arrows) is detected by black nuclear labeling for apoptosis and red cytoplasmic staining for CD8. Apoptosis of CD8⁺ T lymphocytes (arrows) was most abundant in the EL of allografts placed in WT and eNOS^−/− recipients. The graph (m) shows quantitative T lymphocyte apoptotic activity in both the EL and SEL of the graft. Apoptosis of T lymphocytes was reduced by recipient iNOS deficiency. *, P < 0.05 versus allografts from WT recipients; #, P < 0.05 versus allografts from iNOS^−/− recipients; bar, 50 μm.

Figure 4. — Apoptotic cells were detected using the in situ TUNEL labeling method (a–d; arrows) and further corroborated immunohistochemically by colocalizing caspase-3 expression in the same cells in adjacent sections (e–h; arrows). Double staining for apoptotic CD8⁺ T lymphocytes (i–l; arrows) is detected by black nuclear labeling for apoptosis and red cytoplasmic staining for CD8. Apoptosis of CD8⁺ T lymphocytes (arrows) was most abundant in the EL of allografts placed in WT and eNOS^−/− recipients. The graph (m) shows quantitative T lymphocyte apoptotic activity in both the EL and SEL of the graft. Apoptosis of T lymphocytes was reduced by recipient iNOS deficiency. *, P < 0.05 versus allografts from WT recipients; #, P < 0.05 versus allografts from iNOS^−/− recipients; bar, 50 μm.