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(A) RT-PCR using specific primers for c-fos. Cells were treated for 30 minutes with 50 mM KCl or treated with NaCl as control, in the presence or absence of FK (100 nM) and cyclosporin A (300nM). (B) Time course of c-fos and IP3R1 mRNA expression determined by RT-PCR. Cells were depolarized with 25 mM KCl on day 2 and harvested after different times.
