Table I.
| Colony formation assay
|
Plating efficiency of stable NIH-3T3–derived cell lines
|
|||
|---|---|---|---|---|
| Vector | Colony number (SD) | Cell line | Inoculated cells | Colony number (SD) |
| pMX | 0 (0) | NIH-3T3 | 106 | 0 (0) |
| pMX + WT-STAT3 | 0 (0) | NIH-3T3-core | 106 | 0 (0) |
| pMX + STAT3-C | 8 (4) | WT-STAT3 | 106 | 0 (0) |
| pMX-core | 0 (0) | WT-STAT3/core | 104 | 110 (32) |
| pMX-core + WT-STAT3 | 10 (4) | STAT3-C | 104 | 92 (26) |
| pMX-core + STAT3-C | 20 (10) | STAT3-C/core | 104 | 222 (42) |
| v-src | 96 (22) | v-src | 103 | 180 (34) |
NIH-3T3 cells were infected with pMX or pMX-core viruses and then transfected with WT-STAT3, STAT3-C, or v-src. Transfected cells (106) were plated in soft agar. 3 wk later, the colony number was determined. Results shown are the mean ± SD of 3–8 experiments. The plating efficiency of representative cell lines derived from soft agar or retrovirus infection (NIH-3T3-core) or clones selected in G418 (WT-STAT3) was determined by plating the indicated number of cells into soft agar and counting the colonies after 10 d.