Figure 1.
SSCP analysis from two CLL cases displaying either the absence or presence of intraclonal diversification. All 27 VHDJH transcripts from CLL 216 showed identical mobility (A). In CLL 105, seven of the 25 VHDJH transcripts showed a mobility pattern different from that displayed by the remaining 18 VHDJH transcripts (B). Sequence analysis showed that the VHDJH cDNAs 1–3 were all collinear and collinear with the most represented transcripts confirming their monoclonality. However, these transcripts displayed nucleotide variations distributed randomly throughout the VH segment. VHDJH transcripts labeled 1 were identical among themselves but different, though collinear, from the VHDJH transcripts 2 and 3, and the most represented transcripts. VHDJH transcripts 2 were all identical but different, though collinear, from the VHDJH transcripts 1, 3, and the most represented transcripts. VHDJH transcripts 3 were identical but different, though collinear, from the VHDJH transcripts 1, 2 and the most represented transcripts.