Table I.

Administration of Anti–IL-10 mAb Blocks Exacerbation of Anemia in Fas-deficient H+L6 Homozygous Mice

Treatments	Hematocrit values (%)	Amounts of the autoAb bound to RBCs (mean fluorescence intensity)	Ig-producing cells (per 105 cells)
Anti–IL-10	37.5 ± 2.5a	494.4 ± 364.2b	235.8 ± 173.1b
Control Ig	31.5 ± 0.5	1,419.2 ± 497.2	660.6 ± 240.2

Either anti–IL-10 mAb or control rat IgG (100 μg/injection) was injected weekly into Fas-deficient H+L6 homozygous mice. 4 wk after the first injection, hematocrit values (%) were measured. Amounts of the autoAb bound to RBCs were assessed as mean fluorescence intensity of FITC-conjugated anti-IgM Ab bound to RBCs. Numbers of anti-RBC Ab-producing cells in MLN were measured by ELISPOT assay using anti-IgM Ab. Values represent the mean ± SD of five mice in each experimental group. Paired t test values calculated between anti–IL-10 and control rat IgG.

^a P < 0.01.

^b P < 0.02.