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. 2002 Jul 1;196(1):87–95. doi: 10.1084/jem.20012084

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Proliferation profile and cytokine production of T cells from Bcl-xγ wt and −/− mice after CD3 and CD28 ligation. (A) Proliferation of activated T cells from wt and −/− mice. T cells were isolated from wt and −/− mice and cultured for 48 h in the presence of plate-bound anti-CD3 and/or anti-CD28 antibodies. Cells were then pulsed with ³[H]thymidine and cultured for an additional 18–20 h before harvesting. Data (±SEM) represents the mean thymidine uptake of triplicate cultures as described in Materials and Methods. (B) Time-course of IL-2 production from activated T cells. Supernatants from T cells activated as described in (A) were harvested at the indicated times and analyzed for IL-2 by ELISA. Values are the average of triplicate determinations (±SE) from one of three representative and independent experiments as described in Materials and Methods.

Figure 2. — Proliferation profile and cytokine production of T cells from Bcl-xγ wt and −/− mice after CD3 and CD28 ligation. (A) Proliferation of activated T cells from wt and −/− mice. T cells were isolated from wt and −/− mice and cultured for 48 h in the presence of plate-bound anti-CD3 and/or anti-CD28 antibodies. Cells were then pulsed with ³[H]thymidine and cultured for an additional 18–20 h before harvesting. Data (±SEM) represents the mean thymidine uptake of triplicate cultures as described in Materials and Methods. (B) Time-course of IL-2 production from activated T cells. Supernatants from T cells activated as described in (A) were harvested at the indicated times and analyzed for IL-2 by ELISA. Values are the average of triplicate determinations (±SE) from one of three representative and independent experiments as described in Materials and Methods.