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. 2002 Jul 1;196(1):1–13. doi: 10.1084/jem.20020268

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Positive selection occurs less efficiently in TCRs lacking the CD3γ–ITAM. Day 14 fetal thymic lobes of WT and CD3γ–ΔITAM mice were cultured in FTOCs, and analyzed over time for the generation of thymocytes expressing TCRβ (A and B), and CD4 and CD8 (C). A histogram for the TCRβ staining on day 7 of the FTOC is shown in panel A, as well as the gates for distinguishing TCRβ-hi and TCRβ-lo cells. A time course for the development of cells expressing high levels of TCRβ is shown in B, and the percentages of DN, DP, and SP cells on day 7 of the FTOC are shown in C. For SP cells, only those also expressing TCR are shown. Data are representative of three separate experiments.

Figure 3. — Positive selection occurs less efficiently in TCRs lacking the CD3γ–ITAM. Day 14 fetal thymic lobes of WT and CD3γ–ΔITAM mice were cultured in FTOCs, and analyzed over time for the generation of thymocytes expressing TCRβ (A and B), and CD4 and CD8 (C). A histogram for the TCRβ staining on day 7 of the FTOC is shown in panel A, as well as the gates for distinguishing TCRβ-hi and TCRβ-lo cells. A time course for the development of cells expressing high levels of TCRβ is shown in B, and the percentages of DN, DP, and SP cells on day 7 of the FTOC are shown in C. For SP cells, only those also expressing TCR are shown. Data are representative of three separate experiments.