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. 2002 Jul 1;196(1):1–13. doi: 10.1084/jem.20020268

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — F5–ΔITAMγ mutant receptors are fully capable of transmitting signals for negative selection in response to ASNENMDAM, but are impaired in triggering signals for positive selection. Purified DP thymocytes of F5–WT (open circles) and F5–ΔITAMγ mice (filled circles) were incubated overnight in the presence of LEC cells as APCs with the indicated concentrations of the nominal peptide ASNENMDAM and seven of its variants, or with the null-peptide ASNANMDAM (bottom right panel) as a negative control. Cells were analyzed (A) for the percentage of CD8 SP thymocytes as a readout system indicative for induction of positive selection or (B) cell death by propidium iodide staining as a readout system indicative for induction of negative selection. Data shown is presented as the percentage of response calculated as described in Materials and Methods.

Figure 6. — F5–ΔITAMγ mutant receptors are fully capable of transmitting signals for negative selection in response to ASNENMDAM, but are impaired in triggering signals for positive selection. Purified DP thymocytes of F5–WT (open circles) and F5–ΔITAMγ mice (filled circles) were incubated overnight in the presence of LEC cells as APCs with the indicated concentrations of the nominal peptide ASNENMDAM and seven of its variants, or with the null-peptide ASNANMDAM (bottom right panel) as a negative control. Cells were analyzed (A) for the percentage of CD8 SP thymocytes as a readout system indicative for induction of positive selection or (B) cell death by propidium iodide staining as a readout system indicative for induction of negative selection. Data shown is presented as the percentage of response calculated as described in Materials and Methods.