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. 2002 Jul 1;196(1):1–13. doi: 10.1084/jem.20020268

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Copyright © 2002, The Rockefeller University Press

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Figure 7. — Activation of LAT and ERK but not JNK is strongly reduced in the absence of a signaling deficient CD3γ chain. DP thymocytes from 6–8-wk-old F5–WT and F5–ΔITAMγ mutant mice were stimulated with cross-linked anti-TCRβ (A) or the agonist peptide ASNENMDAM and anti-CD28 (B and C). At the indicated times, induction of ERK, LAT, and ZAP70 phosphorylation was assessed by immunoblotting of whole-cell lysates or ZAP70 immunoprecipitates (A and B), while JNK activity was measured using an in vitro kinase assay (C). JNK and ZAP70 experiments were performed only with anti-TCRβ. Protein loading was analyzed by immunoblotting with anti-ERK, anti-LAT, anti-ZAP70 (A and B), or anti-JNK (C) mAbs.

Figure 7. — Activation of LAT and ERK but not JNK is strongly reduced in the absence of a signaling deficient CD3γ chain. DP thymocytes from 6–8-wk-old F5–WT and F5–ΔITAMγ mutant mice were stimulated with cross-linked anti-TCRβ (A) or the agonist peptide ASNENMDAM and anti-CD28 (B and C). At the indicated times, induction of ERK, LAT, and ZAP70 phosphorylation was assessed by immunoblotting of whole-cell lysates or ZAP70 immunoprecipitates (A and B), while JNK activity was measured using an in vitro kinase assay (C). JNK and ZAP70 experiments were performed only with anti-TCRβ. Protein loading was analyzed by immunoblotting with anti-ERK, anti-LAT, anti-ZAP70 (A and B), or anti-JNK (C) mAbs.