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. 2002 Jul 1;196(1):27–38. doi: 10.1084/jem.20011682

Figure 1.

Figure 1.

A 65-kD isoform of Stat6 is produced by proteolytic processing. (A) Cell extracts from WT splenocytes were incubated with cell extracts of BMMCs from Stat6−/− mice at 37°C for 20 min and analyzed by Western blotting with anti-Stat6 (M200) antibody (top) or anti-Stat6 (M20) antibody (bottom). As controls, cell extracts from WT BMMCs and Stat6−/− BMMCs were blotted with anti-Stat6 antibodies. Representative blots from four independent experiments are shown. (B) COS7 cells were transfected with Stat6 expression vector and their cell extracts were used as a source of Stat6 protein. Transfected Stat6 was incubated with cell extracts of thymocytes, splenocytes, or BMMCs from Stat6−/− mice at 37°C for 20 min and analyzed by Western blotting with anti-Stat6 (M200) antibody. A representative blot from four independent experiments is shown.