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. 2002 Jul 1;196(1):27–38. doi: 10.1084/jem.20011682

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — BMMC extract cleaves Stat6 but not Stat5. (A) Cell extracts from IL-3–stimulated WT BMMCs were blotted with either anti–phospho-Stat5 (top) or anti–pan-Stat5 (bottom) antibody. (B) Either murine Stat5a (top) or murine Stat6 (bottom) from transfected COS7 cells was incubated with nuclear extract from either WT BMMCs or FDC-P1 cells. Reactions were separated on 6 or 10% polyacrylamide gels and subjected to Western blotting with anti-pan Stat5 antibody (top) or anti-Stat6 (M200) antibody (bottom).