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. 2002 Jul 1;196(1):27–38. doi: 10.1084/jem.20011682

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — Stat6 is cleaved between aa 673 and 695 by the Stat6 protease and the resultant Stat6 functions as a dominant-negative molecule. (A) COOH-terminal–truncated mutants of Stat6 (715, 695, or 673 Stat6) were transfected to COS7 cells. Cell extracts from these cells were incubated with or without Stat6^−/− BMMC extract and blotted with anti-Stat6 (M200) antibody. A representative blot from four independent experiments is shown. (B) COS7 cells were transfected with 695, 673, or WT Stat6 (as a control) in the presence of a Stat6-responsive reporter construct, TPU474. 20 h after transfection, cells were stimulated with or without IL-4 for an additional 12 h and the luciferase activity of TPU474 was measured by the dual luciferase reporter system. Data are means ± SD for four experiments. (C and D) Similar to B, COS7 cells were transfected with WT Stat6 in the presence of the indicated amounts of either (C) 695 or (D) 673 Stat6. Data are means ± SD for four experiments.