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. 2002 Jul 1;196(1):27–38. doi: 10.1084/jem.20011682

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Copyright © 2002, The Rockefeller University Press

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Figure 7. — Stat6 protease regulates the nuclear accumulation of Stat6 in mast cells. (A) CFTL-15 cells were infected with either MSCV-C-Flag WT Stat6-IRES-Thy1.1 retrovirus or MSCV-C-Flag D685A Stat6-IRES-Thy1.1 retrovirus and infected (Thy1.1⁺) cells were positively collected by magnetic cell sorting. Before and after IL-4 stimulation, subcellular localization of transfected Stat6 was visualized by Cy3-conjugated anti-Flag antibody. Their nuclei were stained with 4′-6-Diamidino-2-phenylindole. Where indicated, 10 ng/ml leptomycin B was added to the culture to block nuclear export. DAPI, 4′-6-Diamidino-2-phenylindole; LMB, leptomycin B. (B) COS7 cells were transiently transfected with either C-Flag WT Stat6 or C-Flag D685A Stat6 expression plasmids. Before and after IL-4 stimulation, subcellular localization of transfected Stat6 was visualized by Cy3-conjugated anti-Flag antibody.