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. 2002 Jul 1;196(1):27–38. doi: 10.1084/jem.20011682

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Copyright © 2002, The Rockefeller University Press

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Figure 9. — Retrovirus-mediated expression of D685A Stat6 enhances IL-4–induced growth inhibition of Stat6^−/− BMMCs. (A) Stat6^−/− BMMCs were infected with retroviruses of pMX-WT Stat6-IRES-GFP, pMX-D685A Stat6-IRES-GFP, or pMX-IRES-GFP, and then cultured with IL-3 alone (left) or IL-3 plus IL-4 (right). The number of live infected BMMCs (GFP⁺ c-kit⁺ PI⁻ cells) was determined at days 0, 3, and 7 using a hemocytometer and FACS^®. Data are means ± SD for five experiments. *, significantly different from the mean value of pMX-WT Stat6-IRES-GFP infected cells; P < 0.001. (B) WT BMMCs were infected with retroviruses of pMX-673 Stat6-IRES-GFP or pMX-IRES-GFP and then cultured with IL-3 alone (left) or IL-3 plus IL-4 (right). The number of live infected BMMCs was determined as described above. (C) Stat6^−/− BMMCs were doubly infected with GFP-labeled retroviruses (either pMX-673 Stat6-IRES-GFP or pMX-IRES-GFP) and Thy1.1-labeled retroviruses (either MSCV-C-Flag D685A Stat6-IRES-Thy1.1 or MSCV-IRES-Thy1.1), and then cultured with IL-3 alone (left) or IL-3 plus IL-4 (right). The number of live doubly infected BMMCs (GFP⁺ Thy1.1⁺ PI⁻ cells) was determined at day 0, 3, and 7 using a hemocytometer and FACS^®. Data are means ± SD for four experiments. *, Significantly different from the mean value of pMX-IRES-GFP and/or MSCV-C-Flag D685A Stat6-IRES-Thy1.1 infected cells; P < 0.01.