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. 2002 Jul 1;196(1):97–108. doi: 10.1084/jem.20020298

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — BCR-mediated B7h down-regulation occurs through Ca²⁺ signaling, whereas IL-4–mediated down-regulation occurs through a distinct Stat6-dependent signaling pathway. (A) B7h down-regulation induced by BCR signaling is Ca²⁺ dependent. Purified B cells were treated for 24h with PMA alone, ionomycin alone, or a combination of both and stained for B7h expression. (B) B7h down-regulation by BCR signaling is CsA sensitive. Purified B cells were activated with PMA and ionomycin, anti-IgM F(ab′)₂, IL-4, or untreated either in the presence (solid lines) or absence (solid filled) of CsA. After 24 h of stimulation, cells were stained for B7h expression compared with control staining (shaded histogram). (C) IL-4–mediated B7h down-regulation is Stat-6 dependent. Purified B cells from Stat6^−/− and wild-type control mice were either untreated or activated with IL-4, anti-IgM F(ab′)₂ and IL-4, or PMA and ionomycin. Cells were stained for B7h expression after 24 h of culture.