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. 2002 Oct 7;196(7):999–1005. doi: 10.1084/jem.20020666

Figure 3.

Figure 3.

Retrovirally transduced caspase-8 is able to correct the AICD defect observed in Stat1−/− T cells. (A) CD4+ T cells were purified from pooled spleen and lymph node suspensions obtained from either C57BL-6 (wt), or Stat1−/− mice. These cells were activated and infected in vitro with bicistronic retroviral vectors encoding caspase-8 (two different clones, 8–7 and 8–15) or empty vector (pMIG). The infected cells were then incubated on anti-CD3 coated plates for 20 h to induce AICD, and apoptosis was assayed in GFP+ cells by flow cytometry. The data presented here were obtained from averaging the results of triplicate wells, from one experiment representative of three. (B) The specificity of the requirement for caspase-8 was determined by retrovirally transducing CD4+ T cells from either C57BL/6 (wt), or Stat1−/− mice with viruses encoding caspase-3 (Casp3), caspase-8 (Casp8), or caspase-9 (Casp9), and processed as in A. (C) The levels of caspase-8 expression in retrovirus-infected activated C57BL/6 (WT) and Stat1−/− cells were verified by Western blot analysis.