TABLE 1.
Strains used in this study
| Name | Genotypea | Source or reference |
|---|---|---|
| BUM012 | cynT1 glnV44(AS) gal-3 malT1 xylA7 mtlA2 thi-1 | CGSCb |
| EDCM367 | MG1655 ΔlacZY | 24 |
| EDCM421 | MG1655 ΔlacZY araBAD< >can | This study |
| EDCM464 | MG1655 ΔlacZY can< >FLK2 araBAD< >can | This study |
| EDCM636 | MG1655 ΔlacZY can< >FLK2 | This study |
| EDCM637 | MG1655 ΔlacZY Δcan | This study |
| EDCM638 | MG1655 ΔlacZY can< >FLK2 rpoS::Tn10 (by transduction from RH90) | This study |
| MG1655 | Sequenced λ− and F− derivative of K-12 | CGSC |
| RH90 | MC4100 rpoS359::Tn10 | 21 |
| SM1 | BL21(λDE3) can<>FLK2/pcam-AC (by transduction from EDCM464) | This study |
a
The FLK2 cassette contains two FRT sites flanking a lacZ reporter gene, followed by the aph gene for kanamycin resistance. “<>” indicates replacement.
b
CGSC, Coli Genetic Stock Center at Yale University (http://cgsc.biology.yale.edu/).