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. 2002 Oct 21;196(8):1025–1037. doi: 10.1084/jem.20020760

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — Inflammatory exudates from mice treated with ASA generate novel compounds: LC-MS-MS-based lipodomic analysis. (A) TNF-α–induced leukocyte exudates from dorsal air pouches. Samples were collected at 6 h from FVB mice given ASA and DHA (Materials and Methods). Selected ion chromatogram (m/z 343) showing the production of 17R-HDHA, 7S-HDHA, and 4S-HDHA. Using the diene UV chromophores for quantitation, 7-HDHA was ∼15% of the exudate materials and was identified using a SIM trace for m/z 141 with ms/ms 343. In some exudates 17S-HDHA was also present from LO-dependent routes (see text). MS-MS for B: 17R-HDHA (m/z 343); C: 7S,17R-diHDHA (m/z 359); and D: 4,11,17R-triHDHA (m/z 375). See text for diagnostic ions. Results are representative of n = 7.

Figure 1. — Inflammatory exudates from mice treated with ASA generate novel compounds: LC-MS-MS-based lipodomic analysis. (A) TNF-α–induced leukocyte exudates from dorsal air pouches. Samples were collected at 6 h from FVB mice given ASA and DHA (Materials and Methods). Selected ion chromatogram (m/z 343) showing the production of 17R-HDHA, 7S-HDHA, and 4S-HDHA. Using the diene UV chromophores for quantitation, 7-HDHA was ∼15% of the exudate materials and was identified using a SIM trace for m/z 141 with ms/ms 343. In some exudates 17S-HDHA was also present from LO-dependent routes (see text). MS-MS for B: 17R-HDHA (m/z 343); C: 7S,17R-diHDHA (m/z 359); and D: 4,11,17R-triHDHA (m/z 375). See text for diagnostic ions. Results are representative of n = 7.