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. 2002 Oct 21;196(8):1025–1037. doi: 10.1084/jem.20020760

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Hypoxic HUVECs treated with ASA generate 17R-HDHA. HUVECs were exposed to TNF-α and IL-1β (both 1 ng/ml) and placed in a hypoxia chamber (3 h). The cells were treated with ASA (500 μM, 30 min) followed by DHA (20 μg/10⁶ cells/10 ml plate) and A23187 (2 μM, 60 min). (A) LC-MS-MS chromatogram of ion m/z 343 shows the presence of 17R-HDHA. (B) MS-MS spectrum (RT 21.2 min) of 17R-HDHA identified by retention time, parent ions, and daughter ions and matched with properties and authentic NMR qualified standard.