Table I.

Impact of NSAIDs on COX-2 Conversion of DHA

NSAID	Percentage of inhibition of 13-HDHA	Percentage of increase of 17-HDHA
	%	%
ASA	85.7 ± 5.6	97.8 ± 2.0
Indomethacin	89.8 ± 0.5	0.0
Acetaminophen	87.7 ± 4.3	0.0
NS398 (COX-2 inhibitor)	97.3 ± 1.0	0.0

Results are the mean ± SEM, n = 3. Products were extracted, identified, and quantitated using deuterium internal standards and LC-MS-MS (Materials and Methods). NSAIDs were incubated 30 min with human recombinant COX-2 (Materials and Methods). 2 mM ASA, 200 mM indomethacin, 500 mM acetaminophen, and 100 mM NS398 were used.