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(A) Northern blot analysis of equal amounts (2 μg) of total RNA isolated from strains grown to the mid-exponential phase. Hybridization was performed using a DIG-labeled PCR fragment specific for fnbA. The position of the 23S rRNA is marked by a black dot. (B) Detection of FnBP by ligand blotting using biotinylated fibronectin. Cell wall proteins were isolated from bacteria grown to mid-exponential phase and separated on an SDS-5% polyacrylamide gel.
