FIG. 6.

(A) CP5 expression determined by indirect immunofluorescence of strain Newman and its isogenic sae mutant grown to stationary phase at 37°C in CYPG. Bacteria were marked with CP5-specific monoclonal antibodies and stained with Cy3-conjugated anti-mouse antibodies. (B) Percentages of CP5-positive bacteria. Three microscopic fields with about 50 bacteria each were evaluated, and the percentage of CP5-positive bacteria was determined by comparing the number of fluorescent bacteria with the total number of bacteria stained with DAPI (4′,6′-diamidino-2-phenylindole).