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. 2002 Oct 21;196(8):1079–1090. doi: 10.1084/jem.20020991

Figure 2.

Figure 2.

Gastric but not peripheral DCs induce the activation of TXA-23 T cells. (a) Intracellular IFN-γ staining of TXA-23 and DO11.10 cells T cells. TXA-23 and DO11.10 T cells were cultured for 16–18 h alone (no DC) in the presence of isolated gastric or peripheral DCs (+DC), and in the presence of DCs with added H+/K+-ATPase peptide (final concentration 50μg/ml) or OVA peptide (+DC +peptide; final concentration 10μM). For TXA-23 cells, one representative experiment out of ten, and for DO11.10 one out of four, is shown. Markers were set according to isotype-matched negative control staining in this and all similar experiments. (b) The stimulatory capacity of isolated gastric DCs compared with that of peripheral DCs pulsed with graded amounts of H+/K+-ATPase peptide. (c) Surface phenotype of gastric, peripheral, and mesenteric DCs (filled) in comparison to isotype-matched negative control staining (open). IFN-γ responses of TXA-23 cells that were cultured for 16–18 h in the presence of gastric, peripheral, or mesenteric DCs in the absence of added antigen are also shown (one representative experiment out of three).