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. 2002 Oct 21;196(8):1079–1090. doi: 10.1084/jem.20020991

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — Immunofluorescence staining of H⁺/K⁺-ATPase and DCs in the gastric LN and stomach sections of animals with AIG. (a) Stomach sections from untreated BALB/c^nu/nu mice (stomach) and BALB/c^nu/nu mice with AIG (stomach AIG) were stained with hematoxylin and eosin (first and third panel) or with FITC-2G11 (second and fourth panel). (b) Double staining of stomach sections of untreated BALB/c^nu/nu mice (stomach) and BALB/c^nu/nu mice with AIG (stomach AIG) for H⁺/K⁺-ATPase (green) and anti–CD11c-TXRD (red). Laser scanning confocal microscopy was conducted on a similarly stained stomach section from an animal with AIG. One individual optical section in the z-plane is shown with cross-sectional profiles in the y and x axes (third panel). A 3D reconstruction of the whole cell was produced from these image data, which demonstrates the juxtaposition of H⁺/K⁺-ATPase⁺ PCs (green) and a CD11c⁺ DC (red; fourth panel). (c) Gastric LN sections of an untreated BALB/c^nu/nu mouse (gastric LN) and a BALB/c^nu/nu mouse with AIG (gastric LN AIG) were stained with FITC-2G11 (green) and anti–CD11c-TXRD (red). Positive staining with the anti–H⁺/K⁺-ATPase mAb is restricted to CD11c⁺ DCs (circles). (d) Laser scanning confocal microscopy was conducted on a similarly stained gastric LN section from an animal with AIG. One individual optical section in the z-plane is shown with cross-sectional profiles in the y and x axes (left). A 3D reconstruction of the whole cell was produced from these image data (middle) and the intracellular localization of the H⁺/K⁺-ATPase staining (green) was visualized by decreasing the opacity of the CD11c staining (red; right).