TABLE 6.
Packaging behavior of mutants of structural protein P1
| Oligonucleotides used | Base changes
|
Amino acid changes | Packaging | Plaque formation | Second-site revertants | Suppressor(s) | |
|---|---|---|---|---|---|---|---|
| Wild type | Change | ||||||
| 572 and 573 | TGGCGCAAG | GTGGCCAAG | WR103VA | No | No | ||
| 574 and 575 | CGTCGTATG | GCTGCTATG | RR196AA | No | Poor | Yes | T316I, A402T |
| 601 and 602 | GAGCTCGAC | GCGCTCGCC | ELD268ALA | No | No | ||
| 599 and 600 | CGTTTGCGC | GCTTTGGCC | RLR274ALA | No; no procapsids | No | ||
| 505 and 506 | CAGCGTTTC | CGGGCTTTC | QR384RA | No | |||
| 473 and 474 | CGTTTCCTG | AAGTTCCTG | R385K | Yes | Yes | ||
| 475 and 476 | CGTTTCCTG | GCTTTCCTG | R385A | Poor | No | ||
| 576 and 577 | TACTACGCG | GCCGCGGCG | YY486AA | Poor | |||
| 578 and 579 | CTGAGCCGT | CGGCGGCGT | EP544AA | No procapsids | Poor | EA normal | |
| 500 and 501 | AAACGCTAC | GCAGCCTAC | KR600AA | Yes | Poor | Yes | E590K |
| 498 and 499 | CGTCGCGAA | GCTGCCGAA | RR617AA | No | Poor | Yes | Y486C, Y486S |
| 502 and 503 | CGTCGCACG | GCTGCCACG | RR651AA | Yes | Yes | ||
| 562 and 563 | CGTCGTATG | GCTGCTATG | RR666AA | Yes | Yes | ||
| 566 and 567 | CAAAACGCT | GCGGCCGCT | QN669AA | Yes | Yes | ||
| Mpac | GC142AU | Yes | Yes | E101G | |||
| Mpac | GC142AU | Yes | Yes | E133G | |||
| Spac | GC99AU | Yes | Yes | E142G | |||
| Mpac | GC142AU | Yes | Yes | Q384K | |||
| Mpac | GC142AU | Yes | Yes | Q384R | |||
| Spac | GC99AU | Yes | Yes | E390A | |||