Table I.
Lymphocyte Populations in p110δ−/− Mice
| Tissue/cell type | Control | p110δ−/− |
|---|---|---|
| Bone Marrowa | ||
| Fraction A–C | 8.6 × 105 (2.7) | 7.5 × 105 (1.5) |
| Fraction D | 4.1 × 106 (1.3) | 3.1 × 106 (0.8) |
| Fraction E | 9.6 × 105 (4.0) | 7.2 × 105 (1.4) |
| Fraction F | 6.6 × 105 (2.4) | 9.6 × 105 (4.2) |
| Spleen (8–10 wk old) | ||
| Large Mac1+ | 1.5 × 106 (0.2) | 1.2 × 106 (0.3) |
| CD4+ | 2.5 × 107 (0.5) | 2.5 × 107 (0.9) |
| CD8+ | 1.3 × 107 (0.3) | 1.1 × 107 (0.4) |
| CD21hi CD23lo | 3.7 × 106 (0.8) | 0.6 × 106 (0.05) b |
| B220+ | 4.4 × 107 (1.3) | 3.1 × 107 (0.7) |
| IgMhi IgDlo | 3.6 × 106 (1.1) | 2.6 × 106 (0.8) |
| IgMhi IgDhi | 1.1 × 107 (0.3) | 1.1 × 107 (0.2) |
| IgMlo IgDhi | 2.6 × 107 (0.8) | 1.6 × 107 (0.4) |
| Spleen (21-d old) | ||
| IgMhi IgDlo | 2.7 × 106 (0.8) | 106 (0.1) c |
| IgMhi IgDhi | 9 × 106 (1.4) | 2.2 × 106 (0.4) c |
| IgMlo IgDhi | 3.2 × 106 (0.2) | 0.8 × 106 (0.2) d |
| Peritoneum | ||
| B1 total | 106 (0.2) | 0.08 × 106 (0.02) c |
| B1a | 2.4 × 105 (0.2) | 0.1 × 105 (0.02) c |
| B1b | 1.9 × 105 (0.3) | 0.07 × 105 (0.01) c |
| B2 | 5.7 × 105 (2.9) | 4.9 × 105 (3.6) |
Values are given as mean with SD in parenthesis. n = 9 for determination of B cell numbers in the spleen of adult mice; n = 4 for all other determinations, except the mutant peritoneum where n = 3.
a
Fractions assessed using the criteria of Hardy. Significant differences are highlighted in bold, significance assessed by Student's t test.
b
P < 0.02.
c
P < 0.001.
d
P = 0.02.