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. 2003 Jul 21;198(2):249–258. doi: 10.1084/jem.20030315

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Abundant clonotype⁺ CD25⁺ cells in DO11 × RIP-mOVA double transgenic mice. (A) FACS^® profiles of DO11 single positive versus DO11 × RIP-mOVA double transgenic littermates at 7 wk of age. Cells were isolated from the indicated lymphoid tissues and stained with CD4-PERCP, CD25-PE, and KJ-126-APC. Plots are gated on CD4⁺ KJ⁺ cells (except for thymus plots, which are gated on CD4⁺ CD8⁻ KJ⁺ cells) and show the percentage of CD25⁺ cells. (B) Absolute number of CD4⁺ KJ⁺ CD25⁺ cells in the lymphoid tissues of DO11 single positive versus DO11 × RIP-mOVA double transgenic littermates. Data show mean and standard deviation from three mice of each genotype aged 6–7 wk. (C) Peripheral LN cells from DO11 × RIP-mOVA double transgenic mice were stained with various combinations of KJ-126-APC, CD62L-FITC, CD69PE, CD4-PERCP, OX40- biotin, streptavidin-PE, IL-7Rα-PE, and CD25-FITC/PE. For intracellular staining cells were surface stained and then permeabilized and stained with CTLA-4-PE. Expression profiles of each marker are shown for gated CD4⁺ KJ⁺ CD25⁻ versus CD4⁺ KJ⁺ CD25⁺ cells.

Figure 1. — Abundant clonotype⁺ CD25⁺ cells in DO11 × RIP-mOVA double transgenic mice. (A) FACS^® profiles of DO11 single positive versus DO11 × RIP-mOVA double transgenic littermates at 7 wk of age. Cells were isolated from the indicated lymphoid tissues and stained with CD4-PERCP, CD25-PE, and KJ-126-APC. Plots are gated on CD4⁺ KJ⁺ cells (except for thymus plots, which are gated on CD4⁺ CD8⁻ KJ⁺ cells) and show the percentage of CD25⁺ cells. (B) Absolute number of CD4⁺ KJ⁺ CD25⁺ cells in the lymphoid tissues of DO11 single positive versus DO11 × RIP-mOVA double transgenic littermates. Data show mean and standard deviation from three mice of each genotype aged 6–7 wk. (C) Peripheral LN cells from DO11 × RIP-mOVA double transgenic mice were stained with various combinations of KJ-126-APC, CD62L-FITC, CD69PE, CD4-PERCP, OX40- biotin, streptavidin-PE, IL-7Rα-PE, and CD25-FITC/PE. For intracellular staining cells were surface stained and then permeabilized and stained with CTLA-4-PE. Expression profiles of each marker are shown for gated CD4⁺ KJ⁺ CD25⁻ versus CD4⁺ KJ⁺ CD25⁺ cells.