Table I.
Proliferation of KJ+ CD25+ and KJ+ CD25− Cells in Response to Immunization
Percent M2 (CFSE low) | Median fluorescence channel (within M2) | Number KJ+ cells (×10-3) | |
---|---|---|---|
KJ+ CD25− | |||
No immunization (day 3) | 6.34 | 37.8 | 6.32 |
Day 3 after immunization | 71.34 | 54.25 | 22.5 |
Day 6 after immunization | 85.8 | 59.64 | 93.0 |
Day 8 after immunization | 98.56 | 33.76 | 111.36 |
Nondraining LN (day 3) | 11.73 | 32.78 | 1.64 |
KJ+ CD25+ | |||
No immunization (day 3) | 2.54 | 39.24 | 4.35 |
Day 3 after immunization | 63.21 | 77.74 | 12.7 |
Day 6 after immunization | 89.52 | 99.65 | 31.0 |
Day 8 after immunization | 93.63 | 87.32 | 39.9 |
Nondraining LN (day 3) | 10.36 | 42.17 | 2.05 |
The data depicted in Fig. 4 A were quantitated in terms of the percentage of KJ+ cells that were CFSE low, the median fluorescence channel within the divided population, and the absolute number of CD4+ KJ+ cells at each time point.