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. 2003 Jul 21;198(2):305–313. doi: 10.1084/jem.20030323

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — Flt3L expands hematopoietic progenitors. FACS^® analysis of hematopoietic progenitor populations in the bone marrow after 5 and 10 d of s.c. Flt3L injections. Mice injected for 5 and 10 d (not depicted) with PBS were used as a control. (A) HSCs (electronically gated on Lin^−/lo Thy1.1^lo cells). (B) CLPs (gated on Lin^−/lo Thy1.1⁻ IL-7Rα⁺). (C) CMPs (CD34⁺ CD16/32^lo), GMPs (CD34⁺ CD16/32^hi), and MEPs (CD34⁻ CD16/32^lo; gated on Lin^−/lo Sca-1⁻ c-Kit⁺ cells). (D) Pro-B cells (gated on IgM⁻ NK1.1⁻ B220⁺ CD43⁺ cells): pro B (A) are CD24^−/lo 6C3⁻ and pro-B (BC) are CD24⁺. Numbers delineate relative percentages of the according population in total bone marrow.