Figure 3.

Generation of DCs and other hemopoietic lineage cells by Flt3⁺ and Flt3⁻ fractions of CMPs. The Flt3⁺ and Flt3⁻ fractions of CMPs were purified from the BM of Ly5.2 mice and i.v. transferred to lethally irradiated Ly5.1 recipient mice. The donor-derived cells were distinguished as Ly5.2⁺ cells. For DC production, recipient spleens were analyzed 2 wk after precursor transfer. Donor-derived DCs were identified as Ly5.2⁺ CD11c⁺ cells from DC-enriched spleen cell preparations (A). The subsets of donor-derived DCs were further segregated by their expression of CD4 and CD8 (A). For myeloid cell production, the recipient BM cells were analyzed 1 wk after precursor transfer for donor-derived Ly5.2⁺ Mac-1⁺ Gr-1⁺ cells (B). For B cell and T cell production, the recipient spleen and thymus were analyzed 2 wk after precursor transfer for donor-derived Ly5.2⁺ B220⁺ or Ly5.2⁺ CD4⁺ cells (B). The data presented is typical of three such experiments. Each experimental group contained two to three recipient mice.