Table II.

Numbers of DCs, Myeloid Cells, and Lymphoid Cells Generated by Flt3⁺ and Flt3⁻ Fractions of CMPs

	Splenic DCs(×106)	BMmyeloid cells(×106)	Spleen B cells(×106)	Thymic T cells(×106)
Flt3+ CMPs (104)	0.27 ± 0.04	0.26 ± 0.09	3.70 ± 0.50	<0.1
Flt3− CMPs (104)	0.04 ± 0.01	0.12 ± 0.02	<0.1	<0.1

The Flt3⁺ and Flt3⁻ fractions of CMPs were purified and i.v. transferred to lethally irradiated Ly5.1 recipient mice. For DC production by the precursors, splenic DCs were analyzed 2 wk after precursor transfer. For myeloid cell production, the BM cells were prepared 1 wk after precursor transfer from the femoral and tibial bones of the recipient mice and analyzed for donor-derived Mac-1⁺ and Gr-1⁺ cells. For B cell and T cell production, the recipient spleen and thymus were analyzed 2 wk after precursor transfer. The cell numbers presented are the mean ± standard error from three experiments. Each experimental group contained two to three recipient mice.