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. 2003 Jul 21;198(2):223–233. doi: 10.1084/jem.20021825

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — MAIR-II stimulates cytokines and chemokines secretion from macrophages. RAW cells transfected with Flag-tagged MAIR-II cDNA at the NH₂ terminus and peritoneal macrophages were pretreated with anti-CD16/32 (Fcγ receptors) to block Fc binding of mAbs, stimulated with plastic-coated control Ig, anti-Flag, or anti–MAIR-II, and cultured for 48 h. Culture supernatants were harvested, and cytokine's and chemokine's concentrations were measured by ELISA.